The effect of probiotic Lactobacillus rhamnosus GG on Candida albicans and Candida tropicalis biofilm formation: A preliminary study

Lactobacillus rhamnosus are Gram-positive and lactic acid-producing bacteria. Meanwhile, Candida albicans tropicalis opportunistic fungi that cause oral candidiasis. This study aimed to determine the effect of LGG on biofilm formation C. with hypothesis inhibits yeasts. ATCC MYA-4901 13803 were standardised 1x106 cells form a mono-species biofilm. L. GG (LGG) was 1x107 cells, equivalent absorbance 0.5 at OD620nm. The microorganisms cultivated in nutrient broth 96-well plate incubated 37°C for 24 h 48 h. Co-culture developed by combining spp. same well similar concentration as mono-culture. Crystal violet assay conducted assess biomass measured OD620nm wavelength. After hours, polymicrobial biofilms decreased 37.1 ± 9.2%. At it further 44.7 5.9%. For tropicalis, co-culture 16.3 5.9% 35.7 7.6% after incubation, respectively. significantly reduced compared (P<0.05). In conclusion, has antibiofilm activity against tropicalis. However, is needed conclude other species strains.